PSVI-2 - Dietary supplementation of Phyllanthus urinaria ameliorates fatty liver hemorrhagic syndrome in hens

Abstract: The purpose of present study was to investigate the effect of Phyllanthus urinaria supplement on fatty liver hemorrhagic syndrome (FLHS) in hens. Forty 7-week-old ISA hens were randomly divided into four groups (n=10), including control diet group (CON), high cholesterol and low choline diet group (CLC), and CLC with 1% P. urinaria powder group (PU). CLC diet and the supplementation of P. urinaria for six weeks exhibited no impact on feed intake and body weight (P > 0.05). The triglyceride (TG) and the total cholesterol (TC) in plasma showed significantly raised at week 6 (TG: CON=18.86 ± 0.97 mg/dl and CLC=66.45 ± 7.17 mg/dl; TC: CON=130.41 ± 5.87 mg/dl and CLC=308.11 ± 27.97 mg/dl); nevertheless, P. urinaria decreased the triglyceride in plasma (PU=41.19 ± 4.88 mg/dl, P < 0.05), but showed no statical significance on total cholesterol (PU= 259.11 ± 20.88 mg/dl, P > 0.05). The malondialdehyde (MDA) concentration in plasma also showed a lower level in 1% P. urinaria powder group (CLC=64.81 ± 3.71 mg/dl and PU=49.13 ± 4.20 mg/dl, P < 0.05). Under liver macroscopic analysis, PU demonstrated the fewer percentage in hepatic appearance of severe level (CLC=22% and PU=0%). Similarly, the liver microscopic examination (H&E) suggested the PU supplementation reduced the score of steatosis (CLC=2.89 ± 0.11 and PU=2.11 ± 0.16) and blooning (CLC=1.63 ± 0.22 and PU=0.88 ± 0.31) in the hepatocyte. To sum up, this study revealed the hepatoprotective effect of Phyllanthus urinaria and provided a potential strategy for preventing FLHS to improve the welfare in hens.