PSIV-30 - Exploring the prebiotic activities of proanthocyanidins on a platform using the three-dimensionally (3D)-cultured organoids

Abstract: Using antibiotic, the antimicrobial substance active against bacteria and promoting growth efficiency, in feedstuffs and food supply has been attracting great attention due to its side effects and contribution to the antibiotic resistance. Therefore, exploring the alternative substitutes of antibiotic is of great significance and urgent need for human health and animal industries with a sustainable high efficiency. The objective of this study is to evaluate the biological and medicinal activities of the monomers of proanthocyanidins (PAs), such as epicatechin, epigallocatechin gallate, and flavonols such as quercetin, isoquercetrin, and rutin generated from engineered plants. The evaluations were performed in intestinal organoids isolated from ileum of neonatal piglets. The organoids after expended in vitro were incubated with or without the candidate compounds for 24 hours, and then treated with or without polyinosinic-polycytidylic acid (Poly (I:C), 10 µg/mL) for another 24 hours, a synthetic analog of double-stranded RNA (can induce a molecular pattern associated with viral infections). Cell proliferation, barrier function, toll-like receptor pathway and apoptosis as well as organic cation transporters were assessed by measuring the abundance of the corresponding genes. Concentration gradient (0-200 µM) measurements showed that isoquercetrin and epigallocatechin gallate might stimulate the intestinal cell proliferation, promote the uptake of L-carnitine, and increase barrier function and mucin secretion (p < 0.05). Epigallocatechin gallate and epicatechin might affect the inflammation responses via modifying expression of Interleukins but not induce the apoptosis. Interactions between the examined these compounds and Poly (I: C) were not observed (p > 0.05), but the influence on the challenged pattern were tested for some of the measured genes (p < 0.05). Based on the data collected from this in vitro study, we conclude that epigallocatechin gallate is a potential PA monomer with all prebiotic characteristics and its applicable values in feedstuff and food supply should be studied in vivo studies in domestic (food) animals with and without antigen challenges. Supported by NC Biotechnology Center project (1107)2022-3082 and the North Carolina Agricultural Research Hatch Projects 02780.