PSXI-24 - Microencapsulation protected Lactobacillus viability and its function in modulating the intestinal microbiota in newly weaned piglets

Wednesday, July 19, 2023

11:00 AM – 12:00 PM MT

Location: 140 Aztec/110 Galisteo

Author(s)

LL

Linyan Li

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi 330047, China; Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada
FY

Fugui Yin

Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada
XW

Xiaoyin Wang

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi 330047, China; Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada
CY

Chongwu Yang

Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada
HY

Hai Yu

Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada
DL

Dion Lepp

Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada
QW

Qi Wang

Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada
ML

Martin Lessard

Sherbrooke Research and Development Centre, Agriculture & Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada
Sherbrooke, Quebec, Canada
LL

Luca Lo Verso

Sherbrooke Research and Development Centre, Agriculture & Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada
Sherbrooke, Quebec, Canada
MM

Martin Mondor

St-Hyacinthe Research and Development Centre, Agriculture & Agri-Food Canada, St-Hyacinthe, Quebec J2S 8E3, Canada
St-Hyacinthe, Quebec, Canada
chengbo Yang, PhD

Associate Professor
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
Winnipeg, Manitoba, Canada
SN

Shaoping Nie

Professor
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, Jiangxi 330047, China
Nanchang, Jiangxi, China (People's Republic)

Author(s)

JG

Joshua Gong

Guelph Research and Development Centre, Agriculture & Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
Guelph, Ontario, Canada

Presenting Author(s)

Tao Wang, Master of Science (she/her/hers)

Student
University of Manitoba
Winnipeg, Manitoba, Canada

Abstract: Lactobacilli are sensitive to heat, which limits their application as probiotics in livestock production. Lactobacillus rhamnosus LB1 was previously shown to reduce enterotoxigenic Escherichia coli (ETEC) and Salmonella infections in pigs. To investigate its potential in the application, the bacterium was microencapsulated and examined for its survival from feed pelleting and long term storage as well as its function in modulating pig intestinal microbiota. The in vitro studies showed that freshly microencapsulated L. rhamnosus LB1 had viable counts of 9.03 ± 0.049 log₁₀colony forming units (CFU) /g, of which only 0.06 and 0.87 Log of viable counts were reduced after storage at 4 and 22°C for 427 days. The viable counts of encapsulated L. rhamnosus LB1 were 1.06 and 1.54 Log higher in the pelleted and mash feed, respectively, than the non-encapsulated form stored at 22℃ for 30 days. In the in vivo studies, 80 piglets (weaned at 21 days of age) were allocated to five dietary treatments for a 10-day growth trial. The dietary treatments were the basal diet (CTL) and basal diet combined with either non-encapsulated LB1 (NEP), encapsulated LB1 (EP), bovine colostrum (BC), or a combination of encapsulated LB1 and bovine colostrum (EP-BC). The results demonstrated that weaning depressed feed intake and reduced growth rates in pigs of all the treatments during 21-25 days of age; however, the body weight gain was improved during 25-31 days of age in all groups with the numerically highest increase in the EP-BC-fed pigs during 21-31 days of age. Dietary treatments with EP, particularly in combination with BC, modulated pig intestinal microbiota, including an increase in Lactobacillus relative abundance. These results suggest that microencapsulation can protect L. rhamnosus LB1 against cell damage from an high temperature during processing and storage while preserving its beneficial function to the animal host. The adverse effects rising from weaning appeared to be alleviated when BC and L. rhamnosus LB1 were included in the diet, implying the complementary functions of both additives.